Study on the expression of IL-32 in pathological scars and biological effect in cicatrization

Objective To explore the biological contribution of IL-32 in the formative course of hypertrophic scar and keloid.Methods From October 2009 to June 2011,12 cases of keloid tissue;12 cases of hypertrophic scar tissue;24 cases of normal skin were collected at Guangdong Medical College Hospital plastic surgical.Take normal skin as control group,the IL-32 mRNA of tissue are investigated in the specimens from normal skin,hypertrophic scar and keloid tissues using reverse transcription polymerase chain reaction(RT-PCR).Further immunohistochemical technique and western blot are performed to detect the expression and distribution of IL-32 protein in the paraffin embedded tissues samples of normal skin,hypertrophic scar and keloid.Results The expression of IL-32 mRNA was obviously lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively.The result of immunohistochemical technique and Western Blot: the expression of IL-32 protein were both lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively(P0.05).Conclusion The results indicated that IL-32 played an important role in the proliferation of fibroblasts and in the development of pathological scar,the value of further research.