2005•Unpublished venueRequires access
Studies on Liquid Culture Conditions of Agaricus blazei Murrill
Wang Li-Duo
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Abstract
The influence of carbohydrate varieties and concentration, nitrogen varieties and concentration, growth factor varieties and concentration, pH, aeration volume, culture temperature, and culture time on liquid culture of Agaricus blazei Murrill(AbM) were studied by means of twice L_(27)(3~(13)) orthogonal tests. optimum formula for AbM in shaking flask culture was determined, which consist of 3 % of corn starch, 0.8 % of soybean cake meal, and 0.15 % of wheat bran, and its pH was 5.0. The optimum culture conditions are as follows, temperature 30 ℃, velocity of shaking table 120 r/min, fed volume 50 mL/250 mL and culture duration 7 days. Under above conditions, biomass of AbM attain to 15.6 g/L.