Studies on Submerged Culture Process of Agaricus Blazei Murill

In this paper,the nutrient factors of Agaricus Blazei Murill were studied in submerged fermentation,it showed that the effect of single factors was carbon sourse,nitrogen source,C/N ratio,growth factors and inorganic salts.The optimum conditions of fermentations were as follows:initial pH5.5,temperature 26℃, the cultivations were performed in 250ml shake flask containing 50 ml medium, and was inoculated to a concentration of 10%. Under these conditions, the time course of growth in shake flask was determined. The results showed that the maximum extracelluar polysaccharide yield was 3.48g/L,when the mycelium had been cultured about 108 hours in shake flask.