Study on Extraction and Purification of Total Flavones from Bamboo Leaves

The optimum extracting process of total flavones from bamboo leaves was obtained after using L9(34) orthogonal test plan. We selected four elements——solvent type; solvent concentration; material-solution ratio and extracting time to observe their effects on the extraction of bamboo flavones. The orthogonal test results showed that the optimum conditions were: 60%, acetone, 3h, material-solution ratio 1: 40. The major factors affecting the content of total flavones were the solvent concentration and the material-solution ratio. Both of them had a significant effect on the yield (FF0.01). On the basis of these experiments, we used x-5 macroreticuler resin together with polyamide to purify the extraction, and the final content of total flavones was up to 78.97%.