The study on expression and role of vascular endothelial growth factor and its receptor gene in acute focal cerebral ischemia at early stage

Objective To study the expression of VEGF mRNA and FLT 1,FLK 1 mRNA in the acute focal cerebral ischemia at early stage,anf to explor the function of VEGF in the acute focal ischemia. Methods The gene expression of VEGF and FLT 1,FLK 1 in acute focal ischemic tissue of different times after MCAO was assayed by in situ hybridization (ISH). The angiogenesis was observed to explore the correlation of VEGF and FLT 1,FLK 1. Results The results of ISH revealed that the gene expression of VEGF and FLT 1,FLK 1 increased from 3h after the MCAO. The expression of VEGF gene reached its peak at the time of 48h after ischemia,then declined gradually,became normal after about 14d. While the gene of FLT 1,FLK 1 declined more slowly after reaching the peak,returned to the control level at the time of 21d. The increasing expression of VEGF mRNA located mainly in the neurons,neuroglia and vascular endothelial cells of penumbra,while FLT 1,FLK 1 mRNA expressed increasingly mainly in the vascular endothelial cell of peripheral ischemic region,it also expressed in the neurons. The angiogenesis appeared in the peripheral ischemic region from 48h after the ischemia,most evidently at the time of 7d. Conclusion VEGF mRNA and FLT 1,FLK 1 mRNA express in the neuron,neuroglia,and vascular endothelial cell. Their expression promotes the angiogenesis in the penumbra and improves the blood supply.