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Expression of vascular endothelial growth factor and its receptors mRNA after focal cerebral ischemic-reperfusion in rats

Meng Lan-qin

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Abstract

Objective To study the expression of vascular endothelial growth factor (VEGF) and its receptors(VEGFR) mRNA at different time points after focal cerebral ischemic-reperfusion(CIR) in rats. Methods Following the establishment of the of transient ischemia modelof middle cerebral artery in rats by Zea-Longa′s method, the expression of VEGF and its receptors mRNA was measured with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points. Results The expression of VEGF mRNA increased 3 h after CIR,and peaked at 6 h, and then declined, returned to the baseline at 7 d. The expression of Flt-1 and Flk-1 mRNA was enhanced at 3 h, and reached its peak at 3 d, then declined gradually. Conclusion The expression of VEGF、Flt-1 and Flk-1 mRNA can be induced by focal CIR in rats.

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What this paper is about

Objective To study the expression of vascular endothelial growth factor (VEGF) and its receptors(VEGFR) mRNA at different time points after focal cerebral ischemic-reperfusion(CIR) in rats. Methods Following the establishment of the of transient ischemia modelof middle cerebral artery in rats by Zea-Longa′s method, the expression of VEGF and its receptors mRNA was measured with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points. Results The expression of VEGF mRNA increased 3 h after CIR,and peaked at 6 h, and then declined, returned to the baseline at 7 d. The expression of Flt-1 and Flk-1 mRNA was enhanced at 3 h, and reached its peak at 3 d, then declined gradually. Conclusion The expression of VEGF、Flt-1 and Flk-1 mRNA can be induced by focal CIR in rats.

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Available abstract

Objective To study the expression of vascular endothelial growth factor (VEGF) and its receptors(VEGFR) mRNA at different time points after focal cerebral ischemic-reperfusion(CIR) in rats. Methods Following the establishment of the of transient ischemia modelof middle cerebral artery in rats by Zea-Longa′s method, the expression of VEGF and its receptors mRNA was measured with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points. Results The expression of VEGF mRNA increased 3 h after CIR,and peaked at 6 h, and then declined, returned to the baseline at 7 d. The expression of Flt-1 and Flk-1 mRNA was enhanced at 3 h, and reached its peak at 3 d, then declined gradually. Conclusion The expression of VEGF、Flt-1 and Flk-1 mRNA can be induced by focal CIR in rats.

Key concepts: Messenger RNA, Vascular endothelial growth factor, Receptor, Ischemia, Reverse transcription polymerase chain reaction, Real-time polymerase chain reaction, Vascular endothelial growth factor C, Internal medicine

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Expression of vascular endothelial growth factor and its receptors mRNA after focal cerebral ischemic-reperfusion in rats — Research Paper | ScholarLens