Study of N-acetylcysteine improving microcirculation with severe acute pancreatitis

Objective: To investigate the value of N- acetylcysteine(NAC) promoting pancreatic microcirculation of rats with severe acute pancreatitis(SAP). Methods: 54 rats were randomly divided into three groups : controlled group(n=18), SAP group(n=18)and NAC group(n=18). The rats were supplied by Hebei medical university. 3.5% sodium taurocholate with 0.1mg/100 g dose was injected into pancreatic duct with minimpump to build the SAP rat model.SAP and NAC group was set time point in 6, 12, 24 hours respectively. 100 mg/kg dose of NAC was used in abdominal cavity. Pancreatic microcirculation was evaluated by measuring serum amylase 、IL- 6、TXB2、6- Keto- PGF1α and T/P. The sample of pancreatic head tissue was dyed with HE methods. The rat speciems were recorded by histopathological score. The infomation was processed by spss16.0 software. Results: Control group showed that the normal pancreatic tissue was no edema and effusion; SAP group showed the morphology of pancreatic tissue appear edema, inflammation effusion, harden tissue and hemorrhage necrosis, NAC group showed that pancreatic tissue was slightly swelling and distension. Pathological evaluation showed that the tissue injury, bleeding and necrosis in NAC group was dramatically alleviated than that in SAP group from 6h to 24 h. Pathological scores in NAC group obviously decreased at6 hours than those in SAP group(P0.05) and more obviously decreased at 12 h and 24 h.Pathological scores showed that there was significantly different between two groups(P0.05).Contrast to SAP group, the serum amylase value in NAC group was obviously reduced in the different time point(P0.05). Contrast to SAP group, serum IL- 6 level in NAC group was significantly reduced in the different time point(P0.05). Contrast to SAP group, TXB2 value in NAC group significantly lowered in different time point(P0.05), but 6- Keto- PGF1αlevels no significant change(P0.05). T/P value decreased statistically(P0.05). Conclusion: NAC can alleviate the severity of SAP through improving pancreatic microcirculation. It might be valuable and helpful for the clinical therapy of SAP.