Effect of Different Media and Hormone Proportioning on the In Vitro Culture of Mature Wheat Embryo

[Objective] The study aimed to establish high-efficiency tissue culture regeneration system suitable for the genetic transformation of good wheat varieties.[Method] With mature embryos of wheat varieties Yumai 18 and Yumai 70 as explants,the influences of 2 media and 4 hormone proportioning on the in vitro culture of mature wheat embryo were discussed.[Result] The average induction and differentiation rates of callus on L3 medium were obviously higher than that on MS medium,the average callus rate of 2 tested varieties was over 91.0% and their average differentiation rate was up to 46.4%.Different hormones and their proportioning could induce the differentiation of callus from mature wheat embryo,but among different treatments,there was great difference of differentiation rate(10.5%～50.0%).L3 as differentiating medium with hormone proportioning of 2,4-D 0.1mg/L and 6-BA 1.5 mg/L was more favorable for the differentiation of callus and the average differentiation rate reached 50%.[Conclusion] The two basic media of MS and L3 had higher induction rate of callus from mature wheat embryo with little difference.The influences of different hormone proportioning on the differentiation rate of callus from matural wheat embryo were great.