Orthogonal Test for Optimizing ethanol reflux extraction process of Rhizoma Coptidis in Insomnia Patch

Objective To optimize the technical conditions of the ethanol reflux extraction for Coptis in Insomnia Patch.The extracted yield of berberine alkaloids,which was regarded as the marker of detection in terms of berberine hydrochloride,were determined by ultraviolet spectrophotometry and the general content data determined were used to optimize the extraction processing conditions by orthogonal test of L9(34).Results The optimum extraction conditions was that the medicinal materials were extracted with 4 times of 50% alcohol for three times,every time for 2 hours.The average extraction rate of berberine alkaloids in 3 batches of samples was 8.4%.Conclusions The optimized extraction process was reasonable.