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2013•Zhongguo yaofangRequires access

Content Determination of Baicalin in Baicalin Capsules by HPLC

Yutang Li

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Abstract

OBJECTIVE:To establish a method for the content determination of baicalin in Baicalin capsules. METHODS: HPLC method was adopted. The determination was performed on Acclaim C 18 (250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-0.1% phosphoric acid(47∶53,V/V)at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm,and the column temperature was 30 ℃. RESULTS:The linear range of baicalin was 20.3-203.0 μg/ml(r=0.999 9) with an average recovery of 99.63%(RSD=0.313%,n=6). The RSDs of precision test,reproducibility test and stability test were all lower than 2%. CONCLUSIONS:The method is accurate,reliable,simple and rapid. It is suitable for the quality control of Baicalin capsules.

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What this paper is about

OBJECTIVE:To establish a method for the content determination of baicalin in Baicalin capsules. METHODS: HPLC method was adopted. The determination was performed on Acclaim C 18 (250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-0.1% phosphoric acid(47∶53,V/V)at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm,and the column temperature was 30 ℃. RESULTS:The linear range of baicalin was 20.3-203.0 μg/ml(r=0.999 9) with an average recovery of 99.63%(RSD=0.313%,n=6). The RSDs of precision test,reproducibility test and stability test were all lower than 2%. CONCLUSIONS:The method is accurate,reliable,simple and rapid. It is suitable for the quality control of Baicalin capsules.

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Available abstract

OBJECTIVE:To establish a method for the content determination of baicalin in Baicalin capsules. METHODS: HPLC method was adopted. The determination was performed on Acclaim C 18 (250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-0.1% phosphoric acid(47∶53,V/V)at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm,and the column temperature was 30 ℃. RESULTS:The linear range of baicalin was 20.3-203.0 μg/ml(r=0.999 9) with an average recovery of 99.63%(RSD=0.313%,n=6). The RSDs of precision test,reproducibility test and stability test were all lower than 2%. CONCLUSIONS:The method is accurate,reliable,simple and rapid. It is suitable for the quality control of Baicalin capsules.

Key concepts: Baicalin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Content determination, Reproducibility, Chemistry, Linear range

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