HPLC-fingerprint study on medicinal Folium Fici Microcarpae of Guangxi

HPLC-fingerprint of Folium Fici Microcarpae of guangxi was studied in the paper. By reverse-phase high performance liquid chromatography, the herbal leaves of Ficus microcarpa taken from 10 sites in Guangxi province were analyzed. The analysis was conducted under the following conditions: an Ultimate ODS (5 μm, 4.6 mm × 250 mm) chromatographic column and acetonitrile-0.05% phosphoric acid solution were selected for gradient elution; detection wavelength was at 280 nm; flow rate at 0.5 mL/min; and analysis period of 120 min. Twelve chromatographic peaks were determined through similarity analysis under the chosen chromatographic conditions to identify the characteristic fingerprint peaks of Folium Fici Microcarpae. The method used to establish the fingerprints is deemed of good stability and reproducibility. Not only is it applicable for quality control of Folium Fici Microcarpae, but also an effective means for fundamental study of valid substances.