Detection of 16S rDNA and PIA genes of Neisseria isolated from the patients with male genitourinary tract infections

Objective To detect the 16S rDNA and PIA genes of Neisseria isolated from the patients with male genitourinary tract infections and investigate the gene identification and pathogenesis of Neisseria species.Methods The 16S rRNA and PIA genes and thier sequences of 14 Neisseria species isolated from reproductive tract of 11 patients with male genitourinary tract infections were identified by PCR amplification and nucleotide sequencing.Results Fourteen Neisseria strains contained 2 strains of Neisseria gonorrhoeae,3 strains of Neisseria mucosa,5 strains of Neisseria cinerea,2 strain of Neisseria subflava,1 strain of Neisseria sicca,1 strain of Neisseria lactamica and 1 strain of Neisseria polysaccharea.The accordance rate of the results reported by 16S rDNA and routine bacteriological method was 85.7%.The nucleotide sequence of virulence-associated gene PIA of Neisseria gonorrhoeae was not found in non-gonococcal strains of Neisseria.ConclusionThe accuracy rate of laboratory diagnosis for the infection caused by Neisseria can be improved by combined use of routine bacteriological method and 16S rDNA identification.The PIA gene is not responsible for the pathogenicity of Neisseria species that cause male reproductive tract infection.