Serotyping and molecular typing Salmonella by PFGE

Objective To document the polymorphism of molecular type and serotype of Salmonella isolated from person in preventive physical examination by creating database aided with PFGE.Methods Twenty-one Salmonella stains were identified and serotyped by diagnostic serum.Then the total genomic DNA of the isolates was digested with restriction enzyme Xba I,and run pulsed-field gel electrophoresis(PFGE).The DNA fingerprints of different stains were analyzed by Bionumerics5.1 software to perform clustering.Results The 21 Salmonella strains belonged to three subgroup of O:4(B),O:9(D1),O:3,10(E1) and 8 serotype;S.Derby,accounted for 33.3%(7/21);S.Agona were 19.1%(4/21) and S.Typhimurium 14.3%(3/21);The DNA fingerprints of 18 Salmonella strains belonged to 15 PFGE pattern combinations.Similarity value of these strains was in the range of 53.9%～100%.One serotype Salmonella showed several PFGE patterns combinations.Conclusion PFGE pattern combinations of this study show polymorphism;The PFGE technology is suitable for molecular typing of Salmonella in preventive physical examination.