Isolation and Identification of Pseudorabies Virus

Porcine pseudorabies virus was isolated from the brain of a 2-day old pig.The Balb/c mice were inoculated with the virus and the typical PRV symptoms could be observed.Infected BHK-21 cell line shrunk,aggregated,and detached from the culture system in 48h approximately.The primers which were designed according to the sequence of PRV gE gene published in GenBank could amplify 354 bp fragment from the isolated virus.These evidences indicate that the isolated virus was PRV.