Effects of total saponins of panax notoginseng on osteogenesis of rat bone marrow stromal cells

Objective To investigate the effects of total saponins of panax notoginseng on osteogenic differentiation and osteogenesis of rat bone marrow stromal stem cells(BMSCs).Methods BMSCs were isolated from femur and tibia of rats and cultured in basal medium,osteogenic-induced medium and osteogenic-induced medium containing PNS 100 mg/L.The proliferation of BMSCs was evaluated by MTT assay,the osteogenic differerntiation was determined by alkaline phosphatase(ALP) activity and Alizarin Red S staining.The expression of RANKL was detected by enzyme-linked immunosorbent assay.Results During osteogenic differentiation,the proliferation,ALP activity and Calcium deposit were increased and the expression of RANKL were decreased compared with basal medium,respectively(P0.05).All effects of osteogenic-induced medium on BMSCs were enhanced by PNS.Conclusions The osteogenic differentiation of BMSCs were promoted by PNS and the down-regulation expression of RANKL is the potential mechanism of PNS on osteoclastogenesis.