Effect of Total Saponins of Panax Notoginseng on Expression of Osteoprotegerin/receptor of Zctivator of Nuclear Ractorκ B-ligand in Rat Bone Marrow Stromal Cells

Objective To evaluate the effect of total Saponins of Panax Notoginseng(PNS) on the expression of osteoprotegerin(OPG) /receptor of activator of nuclear factorκB-ligand(RANKL) expression in rat bone marrow stromal cells(BMSCs),and to investigate the mechanism of it on prevention and treatment for osteoporosis.Methods BMSCs were isolated from femur and tibia of adult rats.After 2 passages,BMSCs were divided into 4 groups.PNS groups were treated with different concentrations of PNS(50,100,and 200 mg/L) while in control group,there was no special treatment.7 d after treatment,the expression of OPG and RANKL were evaluated by RT-PCR and Western Blotting.Results In RT-PCR,the ratio of OPG/ RANKL in PNS groups and control group were(0.40±0.07),(0.41±0.08),(0.78±0.11) and(0.23±0.05),respectively.In Western Blotting the ratio of OPG/ RANKL in PNS groups and control group were(0.64±0.13),(0.28±0.09),(0.13±0.03),and(0.25±0.06),respectively.The ratio of OPG/ RANKL were higher in PNS groups than it in control group,with 100 mg/L PNS group is the highest(P0.05).Conclusion Total Saponins of Panax Notoginseng may enhance OPG expression and inhibit RANKL expression of rat bone marrow stromal cells.