2009•Chinese Journal of Trauma and Disability MedicineRequires access

Effects of Total Saponins of Panax Notoginseng on the Proliferation of Bone Marrow Strowal Cells During Differentiating into Osteoblast in Vitro Mediated by P38MAPK Signal Transduction Pathway

Shi-xin Du

Abstract

Objective:To study the effects of P38MAPK signal transduction pathway on the proliferation of bone marrow strowal cells during differentiating into osteoblast in Vitro.Methods:BMSCs were isolated from femur and tibia of rats and cultured in osteogenic-induced medium(as induced group),osteogenic-induced medium containing PNS 100mg/L(as PNS group) and PNS group medium with SB203580(10uM)(as SB203580 group).The Proliferation of BMSCs were evaluated by MTT assay,the osteogenic differerntiation were determined by alkaline phosphatase(ALP) activity and Alizarin Red S staining.Results:During induction differentiation,the proliferation,ALP activity and Calcium deposit in PNS group were increased compared with induced group and SB203580 group(P0.05～P0.01).ALP activity and Calcium deposit in SB203580 group has no significant difference compared with induced group(P0.05).Conclusion:The proliferation and osteogenic differentiation of BMSCs were promoted by PNS,P38MAPK is the potential signal transduction pathway.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Panax notoginseng, Osteoblast, Alkaline phosphatase, In vitro, Signal transduction, Bone marrow, Medicine, Cell biology