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Molecular cloning, sequence analysis of mouse integrin α_4 gene and repairing of its mutation

LI Hai-qi

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Abstract

Objective To clone and analyze the full-length cDNA of mouse integrin α4, and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of α4 gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α4 cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse α4 had a length of 3 099 bp, and encoded a product of 1 032 amino acids. There were 12 bases pairs mutation of α4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse α4 is cloned successfully.

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What this paper is about

Objective To clone and analyze the full-length cDNA of mouse integrin α4, and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of α4 gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α4 cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse α4 had a length of 3 099 bp, and encoded a product of 1 032 amino acids. There were 12 bases pairs mutation of α4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse α4 is cloned successfully.

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Available abstract

Objective To clone and analyze the full-length cDNA of mouse integrin α4, and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of α4 gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α4 cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse α4 had a length of 3 099 bp, and encoded a product of 1 032 amino acids. There were 12 bases pairs mutation of α4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse α4 is cloned successfully.

Key concepts: Complementary DNA, Molecular biology, Biology, Gene, Restriction enzyme, Cloning (programming), Mutation, clone (Java method)

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Molecular cloning, sequence analysis of mouse integrin α_4 gene and repairing of its mutation — Research Paper | ScholarLens