Effects of epigallocatechin-3-gallate on TF/VIIa/PAR2-enhanced proliferation and migration of SW620 cells

Objective To investigate the interference of epigallocatechin-3-gallate(EGCG) on tissue factor(TF)/blood coagulation factor Ⅶa/protease-activated receptor(PAR)2-promoted proliferation and migration of colon cancer SW620 cells.Methods The SW620 cells were treated with different concentrations of EGCG,PAR2 agonist(PAR2-AP) or Ⅶa,and the cell proliferation and migration were analyzed by MTT method and transwell assay,respectively.The mRNA expressions of TF and cystein asparate proteinase(Caspase)-7 were measured by RT-PCR,and the protein expressions of TF and Caspase-7 were detected by chromogenic substrate assays and Western blot,respectively.Results Compared with PAR2-AP or Ⅶa alone,the cell proliferation,cell migration,expression of TF mRNA and TF activity were all inhibited,whereas the expressions of Caspase-7 mRNA and protein were increased after treated with EGCG+PAR2-AP or EGCG+Ⅶa(P0.05).Conclusion EGCG can affect the expressions of TF and Caspase-7 in SW620 cells and suppress TF/Ⅶa/PAR2-promoted cell proliferation and cell migration.