Inhibitory effects of epigallocatechin-3-gallate on activation of THP-1 cells induced by anti-β_2GPI/β_2GPI complex

Objective To explore the interference effects of epigallocatechin-3-gallate(EGCG) on anti-β2GPI/β2GPI complex-induced activation of human acute monocytic leukemia cell line THP-1.Methods In vitro,THP-1 cells were treated with EGCG,lipopolysaccharide(LPS) and anti-β2GPI/β2GPI complex.The mRNA expressions of tissue factor(TF) and TNF-α in THP-1 cells were detected by real-time quantitative PCR.The activity of TF was determined by chromogenic assay.TNF-α protein in the cell culture supernatant was measured by ELISA.Results EGCG(50 mg/L) could reduce TF mRNA expression and TF activity in THP-1 cells stimulated by anti-β2GPI/β2GPI complex(t=6.97,3.89,P0.05),and also decrease the level of both mRNA and protein of TNF-α in THP-1 cells induced by anti-β2GPI/β2GPI complex(t=3.23,4.65,P0.05).Conclusion EGCG could intercept the effects of anti-β2GPI/β2GPI complex on the activation of THP-1 cells by decreasing the expression of TF and TNF-α.