Factor VIIa decreases the expression of caspase-3 in colon cancer SW620 cells via the activation of PAR2/ERK/NF-κB by tissue factor

Objective To explore the effects of coagulation factor Ⅶa on the proliferation of colon cancer cells SW620 and the expression of apoptotic molecule caspase-3,as well as the mechanisms involved in the process.Methods SW620 cells were treated with protease activated receptor-2 agonists(PAR2-AP) and coagulation factor Ⅶa respectively,then the proliferation rate of the cell was observed.Meanwhile,the expression levels of caspase-3 protein and mRNA in SW620 cells were detected by western blot and quantitative real-time PCR(QT-PCR),respectively.The specific antibody to tissue factor(а-TF),antagonist for PAR2(PAR2-аAP) and inhibitors for ERK1/2(U0126),NF-κB(PDTC) and p38MAPK(SB203580) were used to investigate the effects of factor Ⅶa on the expression of caspase-3.Results After the treatment of PAR2-AP(100 μmol/L) and factor Ⅶa(10 nmol/L),the growth of SW620 cells was promoted significantly and the expression of caspase-3 protein and mRNA in the cells was down-regulated.The inhibitory effect of factor Ⅶa on the expression of caspase-3 in SW620 cells was obviously reversed by a-TF,PAR2-aAP,U0126 and PDTC,but not by SB203580.Conclusion Factor Ⅶa inhibited the expression of caspase-3 in SW620 cells,then promoted the proliferation and growth of SW620 cells via TF-activated PAR2 and ERK1/2/NF-κB signal pathway.