One-step Solidification with Glutaraldehyde in Preparation of Fluorouracil-loaded Chitosan Microspheres

Fluorouracil-loaded chitosan microspheres were prepared by one-step solidification technique with chitosan as drug carrier,glutaraldehyde as crosslinking agent.The preparation was optimized by single factor test with the appearance and encapsulation efficiency as the indexes.The effects of crosslinking agent concentration and crosslinking time on the in vitro release and swelling ratio of the microspheres were investigated.The structure of the microsphere was analyzed by scanning electron microscopy and infrared spectrum.The drug loading and encapsulation efficiency of fluorouracil-loaded chitosan microspheres were 33.5%and 51.2%with mean diameter of(6.8±1.8)μm,and the burst release at 30 min was 33.5%at 30 min.