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2004•Chinese Journal of OsteoporosisRequires access

Effect of total saponins of Panax notoginseng on proliferation differentiation and osteoprotegerin expression of rat osteoblast

Wu Liping

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Abstract

Objective To assess the effect of total sapanins of Panax notoginseng (PNS) on the prolifera-tion, differentiation and osteoprotegerin (OPG) expression of osteoblact and investigate the mechanism of OPG in osteoporosis. Methods Osteoblasts obtained from the calvariae of newborn rats were cutured in medium with dif-ferent concentrations of PNS. Cell proliferation was detected by microscopy and MTT method, Differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion. OPG expression was quanti-fied by immunohistochemistry. Results PNS in 50 μg/ml obviously increased osteoblast proliferiation, differentia-tion and OPG expression. Conclusion PNS stimulates osteoblast proliferention and differentiation, and promote the expression of OPG

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What this paper is about

Objective To assess the effect of total sapanins of Panax notoginseng (PNS) on the prolifera-tion, differentiation and osteoprotegerin (OPG) expression of osteoblact and investigate the mechanism of OPG in osteoporosis. Methods Osteoblasts obtained from the calvariae of newborn rats were cutured in medium with dif-ferent concentrations of PNS. Cell proliferation was detected by microscopy and MTT method, Differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion. OPG expression was quanti-fied by immunohistochemistry. Results PNS in 50 μg/ml obviously increased osteoblast proliferiation, differentia-tion and OPG expression. Conclusion PNS stimulates osteoblast proliferention and differentiation, and promote the expression of OPG

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Available abstract

Objective To assess the effect of total sapanins of Panax notoginseng (PNS) on the prolifera-tion, differentiation and osteoprotegerin (OPG) expression of osteoblact and investigate the mechanism of OPG in osteoporosis. Methods Osteoblasts obtained from the calvariae of newborn rats were cutured in medium with dif-ferent concentrations of PNS. Cell proliferation was detected by microscopy and MTT method, Differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion. OPG expression was quanti-fied by immunohistochemistry. Results PNS in 50 μg/ml obviously increased osteoblast proliferiation, differentia-tion and OPG expression. Conclusion PNS stimulates osteoblast proliferention and differentiation, and promote the expression of OPG

Key concepts: Panax notoginseng, Osteoprotegerin, Osteoblast, Osteocalcin, Alkaline phosphatase, Chemistry, Internal medicine, Endocrinology

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Effect of total saponins of Panax notoginseng on proliferation differentiation and osteoprotegerin expression of rat osteoblast — Research Paper | ScholarLens