Expression and relationship between PCNA, p 16 and selenium Leve in gastric mucosal lesions with Helicobacter pylori infection

To study the expression and relationship between PCNA,p16 and selenium level in gastric mucosal lesions,the expression of p16 and PCNA were investigated in 114 cases of gastric mucosal lesions by immunohistochemistry. Helicobacter pylori (HP)was examined by both of Warthin-Starry method and rapid urease test(RUT).The level of selenium in gastric mucosal was determined by spectrofluorometer.The rusults showed that the level of selenium between the gastric mucosal lesions infected by HP was difference significantly( P 0 05),except the DYS and GC group( P 0 05),the level of selenium in CSG group increased obviously and decreased with the deterioration of the disease( P 0 05),but higher than that in the normal group( P 0 05).The level of selenium had the negative relationship with the expression of PCNA( r s=-0 98?98, P 0 05)and had the positive relationship with the p16( r s=0 914?3, P 0 05).Compared HP positive group with HP negative group in gastric mucosal lesions,the expression of p16 was lower than that in HP negative group( P 0 01),the expression of PCNA was higher than that in HP negative group( P 0 01),the expression of p16 was correlated significantly with expression of PCNA in gastric mucosal lesions with HP infection( P 0 01),the expression of p16 had the negative relationship with the expression of PCNA( r s=-0 607?5, P 0 01).It concluded the expression of PCNA was promoted directly or indirectly by the infection of HP,and the expression of p16 was suppressed.The concentration of selenium increased in gastric mucosal lesions infected by the HP may not only be a protective response to the increaseing oxidative,but also correlate to the expression of multiple encogenes.