The role of microRNA-24 on S100A8 gene 3'-untranslated region in laryngeal carcinoma

Objective To analyze the regulation of S100A8 gene 3'UTR and its targeting miRNA in laryngeal carcinoma.Methods The wide type and mutation type of S100A8 gene 3'UTR luciferase vectors were constructed.MiRanda and RNA22 software were applied to predict the S100A8 3'UTR targeting miRNA.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and pre-miRNA into Hep2 cells.Results Wide type and mutation type S100A8 gene 3'UTR luciferase vectors were successfully constructed.MiRNA targeting prediction showed that the 3rd bp in 3'UTR contained a miR-24 binding site.The luciferase assay revealed that pre-miR-24 could significantly decrease the luciferase activity of wide type S100A8 3'UTR vector,compared with the control and mutation vector transfection groups(P 0.05).Conclusions miR-24 could regulate the activity of S100A8 gene 3'UTR targetely and negatively.