The role of microRNA-125b on HER2 gene 3'-untranslated region in endometrial cancer

Objective:To identify the regulation of miR-125b to HER2 gene 3'UTR in endometrial cancer.Methods: The wild type and mutation type of HER2 gene 3'UTR luciferase vectors were constructed.Software was applied to predict the HER2 3'UTR targeting miRNAs.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and pre-miRNA into HEC-1B cells.Results: MiRNA targeting prediction showed that the 37th bp in 3'UTR contained a miR-125b binding site.The luciferase assay revealed that pre-miR-125b could significantly decrease the luciferase activity of wild type HER2 3'UTR vector,compared with the control and mutation vector transfection groups,P0.05.Conclusion: MiR-125b could negatively target and regulate the activity of HER2 gene 3'UTR.