The Role of miR-223 on PAX6 Gene 3'-Untranslated Region in Glioblastoma Cells

Objective: To identify the regulation of miR-223 to PAX6 gene 3'-UTR in glioblastoma cells. Methods:Bioinformatics software was applied to predict the PAX6 3'-UTR targeting miRNAs. The wild type and mutation type of PAX6 gene 3'-UTR luciferase vectors were constructed respectively. The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and miR-223 mimics into U251 cells.Results: Bioinformatics software prediction results showed that PAX6 may be a target of miR-223. The luciferase assay revealed that miR-223 mimics could significantly decrease the luciferase activity of wild type PAX6 3'-UTR plasmid,compared with the wild type and mutation plasmid transfection group,respectively. Conclusion: miR-223 could negatively target and regulate the activity of PAX6 gene 3'-UTR.