Study on the Apoptosis of Cervical Cancer Cell by Arsenic Trioxide and the Expression of Fas/FasL

Objective:To study the mechanism of apoptosis on cervical cancer cell lines(Hela and SiHa)by As_2O_3.Methods:The DNA fragmentation analysis was determined by the gel electrophoresis.The cell apoptosis ration was measured by flow cytometry(FCM).Cell immunohistochemical method was given to detect the expression of apoptosis-related protein Fas and Fas ligand.Results:We can observe the typical apoptotic cells in the concentrations of 2.5 μmol/L and 5 μmol/L As_2O_3 after 48 h treatment.DNA Ladder was seen.The result of FCM assay demonstrated:the apoptosis induced by As_2O_3 was time dose-dependent(P0.05).As_2O_3(2.5 μmol/L,5 μmol/L)could significantly upregulate the expression of Fas on the two cell lines(P0.05),5 μmol/L As_2O_3 also up regulated the expression of FasL on Hela cell lines(P0.05).Conclusions:Apoptosis does occur on cervical carcinoma cell lines induced by As_2O_3.The upregulation of Fas maybe one of the main mechanisms of apoptosis on cervical cancer cell lines by As_2O_3.