Isolation and Culture of Mesenchymal Stem Cells By Whole Bone Marrow Culture Method in Rats

Objective To establish an efficient method for isolation and culture of mesenchymal stem cells(MSCs)from rat bone marrow.Methods The MSCs were enriched and expanded by whole bone marrow culture.The morphology of the MSCs was examined and analyzed by light microscopy and the purity and phenotype were analyzed by flow cytometry.The ability of osteogenic and adipogenic differentiation of the MSCs under different induction conditions was assessed.Results The MSCs expanded by whole bone marrow culture were adherent cells with a similar spindle-shaped morphology during different passages.The expression of CD45,CD90 was 1.3%,98.6% respectively.The MSCs could be induced to differentiate into osteoblasts and adipocytes.Conclusion Rat MSCs can be isolated and expanded by whole bone marrow culture,which is an efficient and simple method with high purity and good stability.