Rosa RoxburghiiTratt and Fagopyrum Meissn Extract Inhibits Endothelial Cell Growth and Angiogenesis

[Purpose] To investigate effect of Rosa roxburghiiTratt(CL),and Fagopyrum meissn(FR) extract on cell proliferation and apoptosis in human umbilical vein endothelial ECV-304 cell line and on angiogenesis in chicken embryo allantoids membrane(CAM).[Methods] CL with dose of IC30,FR with dose of IC30,and combination at a half of IC30 were treated to ECV-304 cells.The cell proliferation inhibition rate was detected by MTT assay.Cell apoptosis rate was detected by flow cytometry.The changes of Ki-67,Bax and Bcl-2 mRNA and their protein expression were quantified by RT-PCR and Western blot.The vessel inhibitory rat in CAM treated with 100μg/ml CL,100μg/ml FR and 50μg/ml CL plus 50μg/ml FR were analyzed.[Results] The inhibitory rates of IC30 CL,IC30 FR,and CL,FR combination with half of IC30 were 30.87%±1.2%,32.93%±1.4%,and 43.67%±1.5%,respectively.CL,FR alone or their combination all inhibited cell growth in ECV-304 cells with dose-dependent effect.Compared to CL or FR alone,Ki-67 and bcl-2 mRNA(protein) expression decreased in combination treatment group,but expression of Bax mRNA(protein) increased.CL,FR alone or combination inhibited angiogenesis in chicken embryo allantoids membrane,especially with CL.[Conclusion] CL,FR alone or combination can inhibit cell proliferation and induce apoptosis in ECV-304 cell and inhibit angiogenesis in chicken embryo allantoids membrane.