A experimental study on inhibiting effect of cetuximab on angiogenesis

Objective To study the effect of cetuximab (C225) on anti-angiogenesis in vitro and in vivo.Methods Human umbilical vein endothelial cells (HUVEC) were cultured primarily in vitro.MTT colorimetric assay was used to observe the effect of C225 on the proliferations of hUVEC.Transwell migration assay and out-body tube formation test were used to observe the impact of C225 on the migration and vaso-formed ability of HUVEC,and the rate of C225 inducing hUVEC apoptosis was calculated by flow cytometry.The Chick embryo chorioallantoic (CAM) model was used to check the antiangiogenic effect of C225 in vivo.Results C225 (0.0625～2.0000 μg/ml) inhibited the proliferation of hUVEC by 12.34% to 25.26% after treatment for 48 h. The inhibition rate of migration was 15.51% to 48.68% when treated with C225 (0.0625～1.0000 μg/ml).The inhibition rate of tube formation was 22.91% to 44.71% when treated with C225 (0.0625～1.0000 μg/ml). Moreover,when hUVEC was incubated with C225 0.25 μg/ml and 1.00 μg/ml,the rates of cell apoptosis were 33.20% and 35.05%,respectively.It was observed that C225 suppressed the neovascularization of CAM in vivo with the inhibition rate of 20.39% to 36.18% when treated with C225 (0.0625～1.0000 μg/ml).Conclusion C225 has anti-angiogenic ability in vitro and in vivo.