Effects of Extract from Rosa Roxburghir Tratt or Extract of Fagopyrum Cymosum Meissn on Cell Proliferation and Apoptosis in Human Esophageal Carcinoma CaEs-17 Cell Line

[Purpose] To investigate the effect of CL plus FR on cell proliferation and apoptosis in human esophageal carcinoma CaEs-17 cell line in vitro.[Methods] The cells proliferation inhibition were detected by MTT assay.Cell apoptosis rate was detected by flow cytometry.The changes of Ki-67,Bax and Bcl-2 expression were quantified by RT-PCR and Western blot.[Results] The CaEs-17 cell proliferation was significantly inhibited by CL or FR alone and CL plus FR,with a dose-dependent manner.The inhibitory rate of CL at dose of IC30,FR at dose of IC30 and CL plus FR at a half dose of IC30 was 26.87%±0.29%,25.20%±0.42% and 39.58%±1.07%,respectively.The cells apoptosis rate tended to increasing orders in control,CL,FR and CL plus FR groups,and that in CL,FR group,compared with that in CL plus FR group were significantly difference(P0.05).Compared with the CL and FR alone group,the CL plus FR group's Ki-67 and bcl-2 mRNA(protein) expressions were markedly decreased and the expression of Bax mRNA(protein) was markedly increased.[Conclusion] Both CL and FR can inhibit cell proliferation and induce apoptosis of CaEs-17 cells,and there is a synergistic effect of CL and FR in vitro.