The Silico Cloning and Bioinformatic Analysis of CAPN2 Gene in Yak

Obiective:The yak CAPN2 gene was cloned by Silico Cloning and analysised sequence,The stduy was performed in order to a theoretical foundation for further research of yak CPAN2 gene and meat quality,the structure of CAPN2 gene and expression.Method:The using of human CAPN2 cDNA sequence as a probe screened homology sequenceas in the EST database.The complete sequence that was spliced by CAP3 was analysised with bioinformatics.Result:The yak CAPN2 gene length size was 3 200bp,contained an open reading frame(ORF) of 2 103bp which it encoded 700 amino acids,the CAPN2 nucleotide sequence of between yak and cattle,sheep,horse,pig,mice,chicken,people corresponding consitsency sequences were 99%,97%,90%,87%,85%,79% and 90%.Phylogenetic tree analysis showed that firstly between yak and the cattle CAPN2 gene were belonged to a branch,and then with sheep,pigs were clustered into a group;human and mice were clustered in a class,these two categories are got together for a large class,finaly these togethered with hores and chickens.After protein analysis resulted that gene encoded protein CAPN2 gene of yak formula for molecular C3569H5503N255O1082S26,weight of 79 935.52,theoretical pI 4.86.Conclusion:The nucleotide sequence of the coding region was between yak and cattle,sheep,horses,pigs,mice,chickens,people were quite conservative.The gene encoding the protein was located in the cytoplasm of the water-soluble protein.