Cloning and Sequence Analysis of TLR2 Gene from Yak

To understand the characteristics of the yak innate immune receptors TLR2 gene,the primers were designed according to the published cattle TLR2 gene sequence in the GenBank.TLR2 gene was divided into three sections for amplification,sequencing,splicin and for bioinformatics analysis and prediction.The results of sequence analysis showed that the length of the cloning TLR2 cDNA is 2 620 bp,containing a complete 2 355 bp open reading frame(ORF).It encodes a peptide of 784 amino acids whose calculated molecular weight is 90.024 6ku;PI is 6.46.The results of protein prediction showed that TLR2 protein performs as the hydrophilicity.Sequence homology analysis showed that yak has higher homology with wild yak,cattle,sheep and other species among the 11 sequences,which has the nearest perimeter in the phylogenetic tree.The results showed that TLR2 gene sequence is very conserved in mammals.The successful cloning of TLR2 gene sequence of yak provides further insight into the study on the disease resistance and molecular mechanism of immune response of yak and other plateau animals in Qinghai Tibet Plateau.