Effect of Extraction of Pelagi-margarita on Proliferation and Apoptosis of Human Cervical Cancer Cell Line Caski

[Objective] To explore the effect of extraction of pelagi-margarita(EPM)on the apoptosis of cervical cancer Caski cell line.[Method] The growth inhibition rate of Caski cells was detected by MTT method,and cell apoptosis rate was analyzed by flow cytometry(FCM)using annexinV /propidium iodide(PI)double staining,cell cycle was detected by flow cytometry using PI staining.Morphologic changes of apoptotic cells were observed by fluorescence microscope,expression of intracellular Bcl-2,Bax protein was analyzed by real time PCR.[Result] EPM inhibited the proliferation of Caski cells in a dose-dependent and time-dependent manner(P0.05).After EPM treatment at 0,6,30 and 60 μg/ml for 24 h,the apoptosis rate of Caski cells was 7.6%,7.15%,18.5% and 38.9%,respectively,while the necrosis rate was 0.45%,2.85%,8.55% and 23.2%.The cells showed characteristic apoptotic morphologic changes under fluorescence microscope.Bioenergy vigor at various concentration could down-regulate the expression of Bcl-2 mRNA,meanwhile,up-regulate the expression of Bax mRNA.[Conclusion] EPM inhibits the proliferation in a dose-dependent and time-dependent manner,and induces the apoptosis of Caski cells by down-regulate the expression of Bcl-2 mRNA,meanwhile,up-regulate the expression of Bax mRNA.