2013•Xi'an Jiaotong Daxue xuebaoRequires access
Regulatory effect of trichostatin A on the cell cycle of SGC7901/ADR
Qiao Wen
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Abstract
Objective To observe the effects of trichostatin A(TSA),a histone deacetylase inhibitor,on the growth and gene expression of SGC-7901/ADR cells resistant to gastric cancer drugs and discuss its action mechanisms.Methods The effect of TSA of different concentration on the growth of SGC-7901/ADR cells was detected by MTT colorimetric assay.Ultrastructural alteration of the cells after TSA exposure was observed under the ordinary microscope.The changes in cell cycle and p21 expression after SGC-7901/ADR cells were exposed to TSA was detected by flow cytometry and RT-PCR,respectively.Results TSA could inhibit the growth of multiple-drug resistant(MDR) SGC7901/ADR cells in a dose-dependent manner.Microscopic observation showed that the growth of the cells was inhibited significantly after they were exposed to TSA.Flow cytometry revealed that the gastric cancer cell cycle arrest was at the G0/G1 phase;RT-PCR detected overexpression of p21 after SGC-7901/ADR cells were exposed to TSA,which might be related to the cell cycle arrest.Conclusion TSA inhibits the growth of SGC7901/ADR cells by up-regulating the mRNA expression of P21 gene.