Effect of trichostatin A on drug-resistant stomach carcinoma cell line SGC-7901/ADR

Objective To investigate the effect of trichostatin A(TSA) on the cell growth and the apoptosis of human gastric cancer cell line SGC-7901/ADR,and to further study the mechanism of TSA in the reversal of drug resistance of gastric cancer SGC-7901/ADR cells.Methods ①The human gastric cancer SGC-7901/adriamycin cells were cultured in vitro,and the logarithmically growing cells were collected.Gastric cancer SGC-7901/ADR cells were intervened by TSA at the concentrations of 0,50,100,200,300,400,500 nmol/L.The OD values of resistant gastric cancer cells were detected by tetrazolium salt(MTT) colorimetric assay after intervention for 24,48,72 and 96 h,the inhibitive rates of cell growth were calculated,and appropriate concentration of TSA for resistant gastric cancer cells was selected.②The apoptosis of SGC-7901/ADR cells was observed by flow cytometry(FCM) after intervention with 100,200,300 nmol/L TSA for 48 h.Results ①TSA had an obvious inhibitive effect on growth of SGC-7901/ADR cells.The inhibitive rate increased in a concentration-dependent manner.②Flow cytometry(FCM) analysis showed that the apoptosis rates of resistant gastric cancer cells increased in a concentration-dependent manner after intervention of TSA for 48 h,which were significantly higher than that in blank controls(P0.05).Conclusion TSA can inhibit the cell growth and induce the apoptosis of gastric cancer cells SGC-7901/ADR,which are positively related to the concentration of TSA,and can reverse the resistance of SGC-7901/ADR cells.