Effect of Trichostain A on Human Gastric Cancer Cell SGC-7901 Growth and Expression of Bcl-2,Caspase-3 mRNA

Objective To investigate the effect of trichostain A(TSA) on growth and expression of bcl-2,caspase-3 mRNA and growth of human gastric cancer cell line SGC-7901 in vitro,and to explore its significance.Methods SGC-7901 human gastric cancer cells were cultured in vitro.MTT colorimetric assay was used to determine the proliferation rate of cells,when SGC-7901 cells were cultured in medium containing the TSA in different time and concentrations.The flow cytometry(FCM) method was used to study the apoptosis and cell cycle induced by TSA in SGC-7901 cells.The expression of bcl-2 and caspase-3 mRNA was determined by reverse transcriplase polymerase chain reaction(RT-PCR).Results MTT assay indicated that the inhibitory effect of TSA on the proliferation of SGC-7901 cells was in does-and-time dependent manner.Comparing with the control group,the cell apoptosis was induced by TSA at 48 hours and increased in does-dependent manner(P0.05).RT-PCR assay showed that the expression of bcl-2 mRNA was downregulated and caspase-3 mRNA was upregulated by TSA at 48 hours with concentration increase(P0.05).Conclusion The growth of SGC-7901 cell in vitro is inhibited by TSA and in dose-and-time dependent manner.TSA also can induce cell apoptosis.Maybe its mechanism is through downgulating the expression of bcl-2 mRNA,upregulating the expression of caspase-3 mRNA.