Simultaneous determination of five main components in lonicera japonica thunb by HPLC

Objective To simultaneously determine the contents of five active components in Lonicera japonica Thunb by HPLC method.Methods The separation of five ingredients was achieved on a Agilent Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm)with gradient elution using solvents of acetonitrile and 0.4% phosphoric acid,the flow rate was 1.0 mL/min.The detective wave length was at 350 nm,and the inpouring volume was 10 μL.Results The recovery rate was in the range of 97.5%~100.2%,and the compounds showed good linearity(r≥0.999 3)in a detection range.Conclusion The method is simple,sensitive,accurate and reproducible,it can be used to control the quality of Lonicera japonica Thunb.