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Determination of Rutin in Lonicera japonica Thunb.by HPLC

Liu Lin-yun

Abstract

High performance liquid chromatography(HPLC) was used to determine the content of rutin in Lonicera japonica Thunb.Chromatograph condition are as below: Zorbax RP-C18 Chromatography column(150×4.6 mm,5 μm);mobile phase: acetonitrile: 0.3% phosphonic acid = 15: 85(v/v);flow rate: 0.6 mL/min;λ=300nm.The linearity of calibration curve was in the range of 0.00084 μg/mL-0.00021μg/mL,and the average recovery rate was 97.7% with RSD=1.93%.

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Key concepts: Rutin, Chromatography, High-performance liquid chromatography, Calibration curve, Acetonitrile, Chemistry, Detection limit, Biochemistry