Effect of Zhonglun′e Decoction on the Expression of Inflammatory Cytokines in Rats with Collagen Induced Arthritis

Objective To observe the effect of Zhonglun′e Decoction a Mongolia medicine,on the expression of anti-CⅡantibody,interleukin-1(IL-1),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in rats with collagen induced arthritis (CIA).Methods Eighty Wistar rats were randomized into control group(n=12)and model group(n=68).CIA model was established through subcutaneous injection of typeⅡcollagen.Sixty selected models were randomized into six groups,i.e.the model group,Zhonglun′e Decoction small-dose group,Zhonglun′e Decoction middle-dose group,Zhonglun′e Decoction largedose group and tripterygium group,with 12in each.The Zhonglun′e Decoction small-dose group,middle-dose group and largedose group were given 0.35g/kg,0.7g/kg and 1.5g/kg of Zhonglun′e Decoction respectively.The tripterygium group was given 5mg/kg of tripterygium tablet.The control group and model group were given normal saline in the equal amount.The levels of anti-CⅡantibody,IL-1,IL-6and TNF-αwere detected by enzyme-linked immunosorbent assay(ELISA)after the administration through gastric feeding for 6weeks.Results Comparing with the control group,the levels of anti-CⅡantibody,IL-1, IL-6and TNF-αwas significantly increased in the model group(P0.01).The levels of anti-CⅡantibody and inflammatory cytokine were significantly decreased in the treatment groups.Both large-dose and middle-dose of Zhonglun′e Decoction as well as tripterygium tablet could decrease IL-1,IL-6and TNF-αwith similar effect.Conclusion Zhonglun′e Decoction could treat CIA by regulating immunity and decreasing the content of IL-1,IL-6and TNF-α.