Experimental study of the treatment of acute myocardial infarction in rats with the mobilization of bone marrow stem cells

Objective To study the therapeutic effects of the mobilization of bone marrow stem cells on acute myocardial infarction(AMI)in rats.Methods Six hours after the ligation of left coronary artery,128 survival AMI female SD rats were randomly assigned to:①AMI control,②simvastatin,③granulocyte-colony stimulating factor(G-CSF)and ④simvastatin+G-CSF groups.After 1,2 or 4 weeks of therapy,mobilization effects,hemodynamics and pathological analysis of rat hearts were compared.Result Mobilization effects were superior to other groups in the ④group at every time.CD34 positive mononuclear cells were found at the edge of infarction zone especially in the ④group.There were significant difference in MI size and capillary density among the four groups.Compared with AMI or simvastatin groups,left ventricular end-diastolic pressure(LVEDP)were significantly decreased while the left ventricular systolic pressure(LVSP)and left ventricular pressure maximal rate of rise or fall(dp/dt)were significantly increased(P0.05 or P0.01) in the ③ and ④ groups,especially in the ④ group (P0.01).Conclusion G-CSF,simvastatin and G-CSF+simvastatin are all effective in the treatment of myocardial infarction in rats by mobilizing bone marrow stem cells, and the combination is the best. [