Comparative effects of granulocyte -colony stimulating factor, simvastatin and their combination in treating of myocardial infarction in rats

Objective: To comparative effects of granulocyte-colony stimulating factor (G-CSF), simvastatin and their combination in vascular proliferation after acute myocardial infarction (AMI)in rats. Methods: Six hours after ligating left coronary artery, 80 surviving AMI female SD rats were randomly assigned to: (1)AMI control (n=20), (2) simvastatin (n=20), (3) G-CSF (n=20) and (4) simvastatin+G-CSF groups. After 2 or 4 weeks of therapy with the drugs by gastric gavage or (and) subcutaneous injection, hemodynamic studies were performed, then the rat hearts were fixed and pathologically analyzed. Results: There were significant difference in MI size and capillary density among the four groups. Compared with AMI or simvastatin groups, Left ventricular end diastolic pressure (LVEDP) were significantly decreased while the left ventricular systolic pressure (LVSP) and left ventricular pressure maximal rate of rise or fall (dp/dt) were significantly increased (P0.05 or P0.01). in the (3) and (4) groups, especially in the (4) group (P0.01). Conclusion: G-CSF, simvastatin and their combination all can promote vascular proliferation and decrease MI size after myocardial infarction ,improve hemodynamics and left ventricular function, with the combination superior.