Experimental study of the treatment of the mobilization of bone marrow stem cells in acute myocardial infarction rats

Objective To investigate the effects and mechanism of G-CSF,simvastatin and their combination on the damage and apoptosis of myocardial cells in acute myocardial infarction(AMI) rats.Methods The AMI model was established in SD rats.Twenty-four hours after the ligation of the left anterior descending coronary artery,surviving rats were randomly divided into five groups: MI group,G-CSF group,simvastatin(MI-S) group,G-CSF and simvastatin(MI-GS) group,and sham-operated(SO) group.Then rats in each group were further assigned to three subgroups according to the days(2 d,14 d and 28 d,respectively) of experiment.Immunohistochemistry staining was performed to lable CD34 and VEGF.The apoptotic myocardial cells were quantified by TUNEL staining.Results 1.The percentage of CD34 positive cells of peripheral blood was higher significantly in MI group comparing with that in SO group(P0.05),and it was higher in treatment group than that in MI group(P0.01).2.The level of integrated optical density of VEGF was higher significantly in MI group comparing with SO group(P0.01),and it was higher significantly in treatment group comparing with that in MI group(P0.01).3 The apoptosis index was decreased significantly in treatment group comparing with that in MI group(P0.01),but it was higher comparing with that in SO group(P0.01);and the apoptosis index was decreased in MI-GS group comparing with those in MI-G and MI-S groups(P0.05).Conclusion G-CSF and simvastatin not only promote stem cells mobilizing,but also significantly depress inflammation,and attenuate the apoptosis of myocardial cells;and these effects are reinforced by the combined use of G-CSF and simvastatin.