Study of separation,purification and structural of polysaccharides of morus nigra linn leaves of Xinjiang

In order to separate,purify and study the preliminary structure of polysaccharides from Morus nigra Linn leaves in XinJiang,the polysaccharides was extracted by reflowing.The DEAE-Cellulose-52 and Sephadex G-200 were used to separate and purify it. The composition of polysaccharides was determined by polyamide TLC and GC and. the relative molecular weight was measured by gel filtration. The type of glycoside bond was determined by IR.Crude polysaccharide was isolated by the DEAE-52 and 6 fractions( W1,W2,N1,N2,N3 and N4) was obtained,and then the W1,N1,N2,and N4 fractions were purified by Sephadex G-200,the elution peak was a single symmetrical peak. The polysaccharide was composed of glucose,mannose,galactose,rhamnose,arabinose,xylose by polyamide TLC and GC. The relative molecular weight of W1,N1,N2 and N4 were 71 331 Da,60 049 Da,35 825 Da and 21374 Da respectively. There were typical absorption peaks of polysaccharide and the glycosidic bond of α-type in Infrared spectrum.