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2006•Journal of Food Science and BiotechnologyRequires access

The Preparation,Purification and Structure Analysis of Water-Soluble Polysaccharides from Angelica sinensis

Sun Yuan-lin, GU Xiao-hong, Jian Tang, LI De-yuan

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Abstract

Polysaccharides from Angelica sinensis were extracted with hot water at 80 ℃.They were fractionated by aion-exchange chromatography and gel filtration chromatography respectively.The results showed that W-ASP11 was composed of glucose,with a molecular weight of 380 000;W-ASP12 was composed of galactose and arabinose,with a molecular weight of 19 000;W-ASP2 and W-ASP3 were composed of galactose,arabinose,rhamnose and trace of glucose and mannose,as well as high amount of uronic acid.The substances were considered as pectic polysaccharides from monosaccharide composition and IR spectra. The major fraction W-ASP3 was eluted with a single peak,and the molecular weight of it was determined by HPGPC as(62,000).

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Polysaccharides from Angelica sinensis were extracted with hot water at 80 ℃.They were fractionated by aion-exchange chromatography and gel filtration chromatography respectively.The results showed that W-ASP11 was composed of glucose,with a molecular weight of 380 000;W-ASP12 was composed of galactose and arabinose,with a molecular weight of 19 000;W-ASP2 and W-ASP3 were composed of galactose,arabinose,rhamnose and trace of glucose and mannose,as well as high amount of uronic acid.The substances were considered as pectic polysaccharides from monosaccharide composition and IR spectra. The major fraction W-ASP3 was eluted with a single peak,and the molecular weight of it was determined by HPGPC as(62,000).

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Available abstract

Polysaccharides from Angelica sinensis were extracted with hot water at 80 ℃.They were fractionated by aion-exchange chromatography and gel filtration chromatography respectively.The results showed that W-ASP11 was composed of glucose,with a molecular weight of 380 000;W-ASP12 was composed of galactose and arabinose,with a molecular weight of 19 000;W-ASP2 and W-ASP3 were composed of galactose,arabinose,rhamnose and trace of glucose and mannose,as well as high amount of uronic acid.The substances were considered as pectic polysaccharides from monosaccharide composition and IR spectra. The major fraction W-ASP3 was eluted with a single peak,and the molecular weight of it was determined by HPGPC as(62,000).

Key concepts: Rhamnose, Angelica sinensis, Polysaccharide, Uronic acid, Chemistry, Arabinose, Size-exclusion chromatography, Chromatography

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