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Purification and Structure Analysis of Polysaccharides from Prunella vulgaris Linn

Yuhua Cao

Open publisher page 4 citations

Abstract

Polysaccharide from Prunella vulgaris Linn was extracted with water at 100 ℃.It was fractionated by anion-exchange chromatography and gel filtration chromatography.The results showed that weight average molecular weight of the major portion PLS3 was 8.3×10-5 Da by HPGPC.The primary structure of PLS3 was determined from its IR spectrum.The pre-column derivatization HPLC and GC analysis showed that the polysaccharide from P.vulgaris Linn and PLS3 were both composed of galactose,glucose,mannouse,xylose,arabinose and rhamnose without uronic acid.But the moler percentages of monosaccharides are different.

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What this paper is about

Polysaccharide from Prunella vulgaris Linn was extracted with water at 100 ℃.It was fractionated by anion-exchange chromatography and gel filtration chromatography.The results showed that weight average molecular weight of the major portion PLS3 was 8.3×10-5 Da by HPGPC.The primary structure of PLS3 was determined from its IR spectrum.The pre-column derivatization HPLC and GC analysis showed that the polysaccharide from P.vulgaris Linn and PLS3 were both composed of galactose,glucose,mannouse,xylose,arabinose and rhamnose without uronic acid.But the moler percentages of monosaccharides are different.

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Available abstract

Polysaccharide from Prunella vulgaris Linn was extracted with water at 100 ℃.It was fractionated by anion-exchange chromatography and gel filtration chromatography.The results showed that weight average molecular weight of the major portion PLS3 was 8.3×10-5 Da by HPGPC.The primary structure of PLS3 was determined from its IR spectrum.The pre-column derivatization HPLC and GC analysis showed that the polysaccharide from P.vulgaris Linn and PLS3 were both composed of galactose,glucose,mannouse,xylose,arabinose and rhamnose without uronic acid.But the moler percentages of monosaccharides are different.

Key concepts: Rhamnose, Derivatization, Prunella vulgaris, Polysaccharide, Arabinose, Chromatography, Uronic acid, Xylose

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