Optimized extraction of alkali-soluble polysaccharide of Grifola frondosa by response surface method

The influence of liquid-solid ratio,extraction time and sodium hydroxide concentration on extraction ratio of alkali extraction Grifola frondosa polysaccharide were studied by quadratic regression orthogonal design.These three parameters were analyzed and optimized by Design-Expert experimental software through the regression model.The optimized extraction parameters were obtained as follows: liquid-solid ratio 66∶1,extraction time 33 min,sodium hydroxide concentration 0.29 mol/L.Under that condition,the extraction ratio of alkali-soluble polysaccharide of Grifola frondosa reached 0.70%.