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AFLP Analysis on the Genetic Diversity of Physic-edible A.chinensis Dallas

Fan Qin

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Abstract

[Objective] To study the genetic diversity of the physic-edible A.chinensis Dallas in Dinghu Mountain and Luofu Mountain of Guangzhou.[Methods]The fingerprint of A.chinensis Dallas was established through amplified fragment length polymorphism(AFLP),so as to analyze the genetic diversity parameters of A.chinensis.[Results] 362 clear bands with 293 polymorphic ones were obtained from 4 primer combinations,and the polymorphic bands covered 80.94% of total.Nei′s genetic diversity index was 0.2467,Shannon polymorphic information index was 0.368 7,the gene differentiation index(GST) among the populations was 0.170 1,and the migration number of each generation was 3.157(Nm).[Conclusion] The results showed that AFLP analysis was of high efficiency and suitable for analyzing the genetic diversity of A.chinensis Dallas;Great genetic variation and diversity exists in the population of A.chinensis.

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[Objective] To study the genetic diversity of the physic-edible A.chinensis Dallas in Dinghu Mountain and Luofu Mountain of Guangzhou.[Methods]The fingerprint of A.chinensis Dallas was established through amplified fragment length polymorphism(AFLP),so as to analyze the genetic diversity parameters of A.chinensis.[Results] 362 clear bands with 293 polymorphic ones were obtained from 4 primer combinations,and the polymorphic bands covered 80.94% of total.Nei′s genetic diversity index was 0.2467,Shannon polymorphic information index was 0.368 7,the gene differentiation index(GST) among the populations was 0.170 1,and the migration number of each generation was 3.157(Nm).[Conclusion] The results showed that AFLP analysis was of high efficiency and suitable for analyzing the genetic diversity of A.chinensis Dallas;Great genetic variation and diversity exists in the population of A.chinensis.

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Available abstract

[Objective] To study the genetic diversity of the physic-edible A.chinensis Dallas in Dinghu Mountain and Luofu Mountain of Guangzhou.[Methods]The fingerprint of A.chinensis Dallas was established through amplified fragment length polymorphism(AFLP),so as to analyze the genetic diversity parameters of A.chinensis.[Results] 362 clear bands with 293 polymorphic ones were obtained from 4 primer combinations,and the polymorphic bands covered 80.94% of total.Nei′s genetic diversity index was 0.2467,Shannon polymorphic information index was 0.368 7,the gene differentiation index(GST) among the populations was 0.170 1,and the migration number of each generation was 3.157(Nm).[Conclusion] The results showed that AFLP analysis was of high efficiency and suitable for analyzing the genetic diversity of A.chinensis Dallas;Great genetic variation and diversity exists in the population of A.chinensis.

Key concepts: Amplified fragment length polymorphism, Genetic diversity, Biology, Diversity index, Population, Genetic variation, Botany, Genetics

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