2010•Medicinal plantRequires access

AFLP analysis on genetic diversity of physic-edible Aspongopus chinensis Dallas.

Fan Qin, Zhu GangLi, Xuegang Sun, Hongbing Cai, Lian Zhen-min, Wang Qi-rui

Abstract

[Objective] To study the genetic diversity of physic-edible Aspongopus chinensis Dallas from Dinghu Mountain and Luofu Mountain of Guangzhou.[Methods] The fingerprint of Aspongopus chinensis Dallas was established through amplified fragment length polymorphism(AFLP),so as to analyze the genetic diversity parameters of Aspongopus chinensis Dallas.[Results] 362 clear bands were obtained from 4 primer combinations,293 of which were polymorphic that accounted for 80.94% of all clear binds.The average Nei's genetic diversity index,Shannon polymorphic information index,gene differentiation index(GST) among populations,and migration number for each generation(Nm) were 0.246 7,0.368 7,0.170 1 and 3.157,respectively.[Conclusion] The results showed that AFLP analysis was of high efficiency that suitable for analyzing the genetic diversity of Aspongopus chinensis Dallas population;great genetic variation and diversity were existed in the populations of Aspongopus chinensis Dallas.

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Key concepts: Amplified fragment length polymorphism, Genetic diversity, Diversity index, Biology, Genetic variation, Population, Botany, Genetics